Saving your work using the leica tcs sp5 confocal microscope system. Leica dmi6000 cs bottom port microscope antivibration table. The leica sp5 confocal is a dmi 6000 model inverted microscope with a super z galvo stage for fast z sectioning and prisms for dic imaging. The leica tcs sp5 ii growth confocal system without compromise, the leica tcs sp5 ii confocal system is equipped with the latest technology, offering high sensitivity and superb resolution. Leica microsystems growth confocal is an imaging system for entrylevel budgets that is fully upgradable as your research demands grow. The leica tcs sp5 upright confocal laserscanning microscope is. To maintain information on the most current level, the description of software functions was intentionally omitted from this operating manual. Jan 04, 2018 this video shows how to configure the internal laser power wavelength etc.
Leica tcs sp5 broadband confocal with the first switchable tandem scanner leica tcs sp5 x first white light laser confocal with tunable vis excitation leica tcs sp5 mp leica tcs sp8 sted 3x fast with opo, excitation up to 0 nm and direct superresolution leica tcs sp8 new confocal platform, modular system. The x y and z controls are most useful with the joystick. The system is equipped with an inverted microscope encased with an incubator for livecell imaging. Multicolor confocal superresolution for leica tcs sp8. Key features upright dm6000cs stand with motorised stage superz galvo 3 pmt detectors, 1 transmitted light detector tunable emission filters. The leica tcs sp5 is supplied with the latest version of the licensed las af software. Sequential scanning using the leica tcs sp5 confocal laser scanning microscope this supplemental manual is the second section of a three part leica tcs sp5 user guide edited by donald pottle schepens 149 w dwp mar. The leica white light laser produces a continuous spectral output between the wavelengths of 470 and 670 nm. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. What is a confocal microscope webinar leica microsystems.
Inverted, manual microscope for industrial applications leica dmi5000 m inverted research microscope for materials testing leica dmi6000 b. The components are the microscope body, two automated stages, the scan head, the electrooptical modulator eom, the monitor and computer, and the spectraphysics maitai tunable nearir laser. This system has five lasers generating nine lines for fluorescence excitation 405, 458, 476, 488, 496, 514, 543, 594, 633 nm. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. Modern cleanliness analysis for your quality assurance by leica microsystems duration. Corneal confocal microscopy for detection diabetic neuropathy duration. The system provides the full range of scan speeds at the highest resolut. Mismatch will lead to loss of intensity and resolution. Conducting a crosstalk test using the leica tcs sp5 confocal microscope before continuing, in order to test for crosstalk. How to use leica confocal microscope sp5 external laser. An introduction from your bosssupervisor is not enough the system has 5 detectors. Leica dm2500 upright fluorescent microscope manuals cell. Two of these detectors are very sensitive hyd sp gaaspdetectors.
National chiao tung university, bio photonics lab, bypraveen kumar. Recognition of the controls to customize and manipulate images using the leica tcs sp5 confocal microscope system. Leica sp5 confocal user manual date of first issue. Leica tcs sp5 confocal user manual only people who have had an introduction from kees straatman are allowed to operate the system. Leica tcs sp2 aobs spectral confocal scanner mounted on a leica dm ire2 inverted fluorescent microscope 405, 458, 476, 488, 496, 514, 543, 594, and 633nm laser excitation lines 10 and 20x dry, 40 and 63x oil, and 63x water immersion objectives. Microscopists utilize the scanning stage for setting up multiple zstacks with the marknfind option and for stitching numerous fields into one large image. It provides the full range of scan speeds at high resolution and is equipped for applications such as frap, fret and fcs analysis. Training manual leica tcs sp5 confocal microscope september 2010.
The leica tcs is a universal laser scanning confocal microscope system for the biomedical and materials research environment. This system is based on an inverted leica dmi 6000 cs microscope base with a conventional scanhead equipped with 7 laser lines that provide flexibility for fluorophore excitation and a zgalvo automated stage for zstacks and 3dprojections. Confocal microscope system with a broad range of scan speeds suited for highresolution morphological imaging, as well as multispectral. Image window robarts research institute leica sp5 training manual september 2010. Confocal microscopy facility schepens eye research. Leica sp5 scanning confocal cdb equipment resource. Even smallest series of throughput are possible in manual process steps.
The leica tcs sp ii system makes it possible to image a single, infocus plane horizontal or vertical as well as a series of planes. Leica sp5 inverted confocal microscope with aresonant scanner allowing highspeed confocal imaging and is well suited for livecell imaging and highresolution morphological imaging. Scanning with confocal using the leica tcs sp5 confocal microscope. The confocal microscope system for highest demands in multiuser facilities. The main focus of this operating manual is directed to the safety notes that must be strictly adhered to when working with the leica tcs sp5 and leica tcs sp5 x. Confocal laser scanning microscope leica tcs sp8 duration. Leica dm6000 cfs upright multiphoton microscope equipped with a tcs sp5 mp laser confocal with both galvonomic and resonance scanners and with spectral hyd detectors. Leica tcs sp5 confocal laser excitation lasers excitation wavelength common excitable fluorophores apeak absorbance empeak emission 405 laser diode 405 nm hoechst 33258 a350 em450 acma aminochloromethoxyacridine a430 em474 dapi 4,6diamidino2phenylindole a372 em456 alexa fluor 405 a402 em421. Leica tcs sp5 upright confocal laserscanning microscope. Jan 11, 2008 basic training video for use of the leica confocal microscope at tufts. Anatomy of the leica sp5 confocal microscope anatomy of the leica sp5 confocal microscope 1. The leica sp5 confocal system has eight visible laser lines provided by a multiline argon laser, a diodepumped solid state laser, and orange and red helium neon lasers.
Leica microsystems sets a new standard with the integration of the leica dm6000 cfs fixed stage microscope into the leica tcs sp5 confocal platform. The facility is located in the woodruff memorial research building wmb on the emory campus. Measuring up to five dimensions, including x, y, z, time and. Updated 8272010 leica tcs sp5 confocal microscope 341 h please contact dr. The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. Leica sp5 training manual september 2010 for the argon laser, adjust the slider to 2030%. Leica tcs sp2 aobs spectral confocal scanner mounted on a leica dm ire2 inverted fluorescent microscope. System components overview 1 tcs sp5 scanner 2 main switch board 3 tcs workstation 4 supply unit 5 control panel 6 microscope stand.
Inverted dmi6000 stand with motorized stage tandem scanner. The tcs sp5 is an upright confocal laser scanning microscope suitable for. The configuration window is used to setup the system with respect to the excitation laser lines, beam. Researchers have the option of using three different lasers an argon, krypton, and a heliumneon laser to excite fluorescently. Instructions for the leica sp5 ii laser scanning confocal microscope. Leica sp5 laser scanning confocal microscope ucsf drc. User guide to the ibif leica tcs sp8 mp confocal microscope. The leica lcs software can perform multidimensional image processing, fret, frap, and lambda scanning as well as controlling the motorized leica dm ire2 microscope. This confocal is reserved for primary and joint cdb faculty and their personnel. Microscopy in medicine lab emory school of medicine.
Conducting a sequential scan using the leica tcs sp5 confocal microscope. The following is the details for confocal platform as well as two microscopes. Ultimate optical design and stability with extreme sensitivity and superb image quality. Microscope table passive upright 156504 701 microscope table active upright 156504 715. M stereomicroscopes 73 pages microscope leica dm operating manual 178 pages. Starting the lcs software on the leica tcs sp5 confocal microscope. In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. The tcs sp5 is an inverted confocal laser scanning microscope suitable for. Improve 3d cell biology with digital lightsheet microscopy from leica microsystems duration. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes.
Basic training video for use of the leica confocal microscope at tufts. Fixed tissue imaging live cell imaging zstack time lapse tile scan roi scan. Dmi 4000 cs scanner is mo from confocal ob and eyepiece torized. This video shows how to configure the internal laser power wavelength etc. Consultation with the equipment manager is required before using this microscope.
Leica sp5 laser scanning confocal microscope training session. Make sure you match the immersion media with the mounting media. The advantage of having a stack of serial optical sections. Confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes. Here more realistic results can be achieved due to the intact network. Microscope leica sp5 confocal user manual 65 pages microscope leica m320 user manual 66 pages microscope leica dm2700 m instructions manual 156 pages microscope leica wild m695 service manual. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix. Leica tcs sp5 broadband confocal microscope leica tcs smd fcs. Leica tcs sp5 ii acoustooptical beam splitter products. The leica tcs sp2 upright confocal laserscanning microscope is located in room 170 and can accommodate specimens labeled with nearly all stains except those requiring ultraviolet excitation. A single vertical section, or xzscan, allows you to see your sample as though from the side. To maintain information on the most current level, the description of software functions was intentionally omitted from these operating instructions.
The confocal microscope and accessories the instrument is a leica tcs sp8 mp confocal microscope, equipped for multiphoton confocal microscopy. Operating the dmi 8 microscope stand there are few buttons you need to operate on this new microscope stand as everything can be operateded through the selfexplanatory touchscreen. System sp5 156501121 confocal detector imaging 1565090 mfp. Science lab, the knowledge portal of leica microsystems, offers scientific research and teaching material on the subjects of microscopy. Confocal microscope system with a broad range of scan speeds suited for highresolution morphological imaging, as well as multispectral imaging. Leica tcs sp5 confocal microscope department of chemical. Usermanual for leica sp8 confocal universitetet i bergen. The leica sp5 laser scanning confocal system is used for advanced multichannel fluorescence and livecell applications. The strength of the individual lasers can be adjusted by moving the appropriate vertical slider arrow from 0 to100%.
Leica tcs sp5 ii system overview en pdf, 4 mb leica tcs sp5 iibrochure technical data. With the combination of leica microsystems tcs sp5 confocal imaging instrumentation, single and multiple projects are easy to handle from single images that need to be evaluated at high standardization to 3d image stacks and 4d time series. Leica tcs sp5 confocal louisiana state university health. The microscopy in medicine core mim core at emory university is a core facility providing access to histology and stateoftheart imaging instrumentation on a shareduse basis to faculty, staff and students of the emory division of cardiology and emory department of medicine. Microscope leica sp5 confocal user manual 65 pages microscope leica dmls instructions manual 160 pages microscope leica m user manual. The system provides the full range of scan speeds at the highest resolution with its proven, highefficiency sp detection five channels simultaneously and optional aobs acousto optical bream splitter, the leica tcs. System overview tcs sp5 ii leica tcs sp5 ii, tcs sp5 mp, tcs sp5 x, flim, smd. This leica sp2 platform is equipped with two leica confocal microscopes. Operating microscope 116 pages microscope leica microstar iv manual.
The system provides the full range of scan speeds at the highest resolution with its proven, highefficiency sp detection five channels simultaneously and optional aobs acousto optical bream splitter, the leica tcs sp5 delivers bright, noisefree. Leica dm2500 upright fluorescent microscope manuals. Neurobiological research has a long history not only in measurements of single cells, but also of thick brain slices. The leica tcs sp5 fully covers the main requirements in confocal and multiphoton imaging with optimal overall performance. This microscope can accommodate specimens labeled with all fluorophores including ultra violet dapi. Leica sp5 confocal microscope umass medical school. Leica tcs sp5 ii the only broadband confocal technical documentation living up to life. Conducting a crosstalk test using the leica tcs sp5 confocal microscope. The leica tcs sp5 is supplied with the latest version of the licensed leica application suite advanced fluorescence. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. Leica tcs sp5 confocal this is a very flexible and fast confocal system build with a leica dmi 6000 cs inverted, fully automated microscope with motorized stage, condenser, objective and filter turrets.
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